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KMID : 0881720210360060520
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Journal of Food Hygiene and Safety
2021 Volume.36 No. 6 p.520 ~ p.527
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Toxin Gene and Antibiotic Resistance of Staphylococcus aureus and Bacillus cereus Isolated from Indoor Air in Cafeteria
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Oh Do-Gyung
Jo Ah-Hyeon
Kim Chan-Yeong
Jeong Eun-Sun
Kim Jung-Beom
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Abstract
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In this study, toxin gene and antibiotic resistance of food poisoning strains isolated from indoor air in the cafeteria were analyzed to prevent food poisoning. Staphylococcus aureus (16 strains) and Bacillus cereus (37 strains) isolated from indoor air in child care center were tested. The toxin genes of S. aureus and B. cereus were detected by PCR assay. The antimicrobial susceptibility test followed the disc diffusion method described by the Clinical and Laboratory Standard Institute. The seg and sei toxin genes were detected in 11 of 16 S. aureus strains (68.6%). The nheA and nheB toxin genes were detected in 37 B. cereus strains. In this study, a total of 12 toxin gene patterns of B. cereus were found, among which the nheA-nheB-nheC toxin gene was found to be the most frequent pattern. The result of the antimicrobial susceptibility test of S. aureus revealed 93.8% and 87.5% resistance to ampicillin and penicillin antibiotics, but methicillin resistance S. aureus and vancomycin resistance S. aureus were not detected. All 37 B. cereus tested in this study were resistant to ampicillin and penicillin antibiotics. Based on the result of this study, it was judged that regular ventilation and air quality management were necessary to prevent food poisoning caused by S. aureus and B. cereus contaminated in the indoor air of child care centers.
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KEYWORD
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Food-borne pathogens, Toxin characteristics, Antibiotic resistance, Indoor air, Child care center
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